1Z1L

The Crystal Structure of the Phosphodiesterase 2A Catalytic Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Detector technology	CCD
Collection date	2002-06-15
Detector	CUSTOM-MADE
Wavelength(s)	1.0070
Spacegroup name	C 2 2 21
Unit cell lengths	90.033, 102.549, 81.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 1.700
R-factor	0.209
Rwork	0.206
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f0j
RMSD bond length	0.004
RMSD bond angle	1.142
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNX

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.700
Number of reflections	39346

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	Tris HCl, magnesium chloride, PEG 8000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K