1Z1G
Crystal structure of a lambda integrase tetramer bound to a Holliday junction
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9793,0.9795,0.9709 |
Spacegroup name | P 31 |
Unit cell lengths | 109.759, 109.759, 265.971 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 4.400 |
R-factor | 0.24683 |
Rwork | 0.244 |
R-free | 0.29207 |
Structure solution method | MIRAS |
RMSD bond length | 0.025 |
RMSD bond angle | 2.831 |
Data reduction software | HKL-2000 |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 4.560 |
High resolution limit [Å] | 4.400 | 4.400 |
Number of reflections | 22783 | |
Completeness [%] | 99.7 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 8000, bis-tris-propane, di-ammonium hydrogen phosphate, glycerol, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8000 | ||
2 | 1 | 1 | bis-tris-propane | ||
3 | 1 | 1 | di-ammonium hydrogen phosphate | ||
4 | 1 | 1 | glycerol | ||
5 | 1 | 1 | DTT | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | PEG 8000 | ||
8 | 1 | 2 | bis-tris-propane | ||
9 | 1 | 2 | di-ammonium hydrogen phosphate |