1Z1A
S. cerevisiae Sir1 ORC-interaction domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9793, 0.9795, 0.9688 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.928, 60.928, 159.257 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.24148 |
| Rwork | 0.238 |
| R-free | 0.27424 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.012 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 11994 | |
| Completeness [%] | 98.3 | 91.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | sodium acetate, PEG 400, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
