1Z0K
Structure of GTP-Bound Rab4Q67L GTPase in complex with the central Rab binding domain of Rabenosyn-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-11 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.440, 81.440, 137.639 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.920 |
| R-factor | 0.22538 |
| Rwork | 0.224 |
| R-free | 0.25889 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Rab4 GTPase |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.302 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 42193 | |
| <I/σ(I)> | 27.29 | 4 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 20% PEG 4000, 200mM ammonium fluoride, 50mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
