1YZQ
GppNHp-Bound Rab6 GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-13 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.495, 68.672, 73.102 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.780 |
| R-factor | 0.23237 |
| Rwork | 0.231 |
| R-free | 0.26488 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Polyalanine Rab3a GTPase |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.177 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 20059 | |
| <I/σ(I)> | 27.7 | 6.2 |
| Completeness [%] | 99.4 | 95.1 |
| Redundancy | 6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 278 | 20% PEG 6000, 300mM Magnesium Chloride, 10% glycerol, 50mM HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
