1YYA
Crystal structure of TT0473, putative Triosephosphate Isomerase from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-13 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 87.612, 118.382, 120.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.890 - 1.600 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.18200 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.054 | 0.363 |
| Number of reflections | 82497 | |
| <I/σ(I)> | 12.7 | 4.79 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.6 | 291 | PEG4000, SODIUM CITRATE, TRIS, SODIUM CHLORIDE, pH 5.6, MICROBATCH, temperature 291K |
