1YWH
crystal structure of urokinase plasminogen activator receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-12-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.928, 136.831, 140.536 |
| Unit cell angles | 90.00, 97.27, 90.00 |
Refinement procedure
| Resolution | 24.850 - 2.700 |
| R-factor | 0.24876 |
| Rwork | 0.245 |
| R-free | 0.31526 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.634 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.850 | 2.769 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.575 | |
| Number of reflections | 110121 | |
| <I/σ(I)> | 12.4 | 2.61 |
| Completeness [%] | 99.5 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | ammonium sulfate, imidazole, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 20K |
