1YVU
Crystal structure of A. aeolicus Argonaute
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-07-22 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9788, 0.9790, 0.95 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.150, 100.679, 115.217 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.900 - 2.900 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.29800 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 2.000 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.900 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.111 | 0.472 |
Number of reflections | 15291 | |
Completeness [%] | 99.6 | 98.6 |
Redundancy | 7.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | PEG 550MME, calcium chloride, cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | PEG 550MME, calcium chloride, cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | PEG 550MME, calcium chloride, cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |