1YV5
Human farnesyl diphosphate synthase complexed with Mg and risedronate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.16 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 110.946, 110.946, 70.264 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.340 - 2.000 |
| R-factor | 0.20765 |
| Rwork | 0.205 |
| R-free | 0.24888 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubv |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.531 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.200 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.092 | |
| Number of reflections | 30212 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.7 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 40% PEG 300, 0.1M phosphate-citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
