1YV5

Human farnesyl diphosphate synthase complexed with Mg and risedronate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-29
Detector	ADSC QUANTUM 4
Wavelength(s)	1.16
Spacegroup name	P 41 21 2
Unit cell lengths	110.946, 110.946, 70.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.340 - 2.000
R-factor	0.20765
Rwork	0.205
R-free	0.24888
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubv
RMSD bond length	0.015
RMSD bond angle	1.531
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.200	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.092
Number of reflections	30212
<I/σ(I)>		2.2
Completeness [%]	99.9	100
Redundancy	8.7	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	40% PEG 300, 0.1M phosphate-citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K