1YT5
Crystal structure of NAD kinase from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 131.448, 137.153, 58.253 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.300 |
| R-factor | 0.217 |
| Rwork | 0.213 |
| R-free | 0.28000 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.539 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.358 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.091 | 0.330 |
| Number of reflections | 44304 | |
| <I/σ(I)> | 15 | 3.2 |
| Completeness [%] | 90.1 | 87 |
| Redundancy | 4.8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | Ammonium Sulfate, pH 6.5, VAPOR DIFFUSION, temperature 298.0K |
