1YS4

Structure of Aspartate-Semialdehyde Dehydrogenase from Methanococcus jannaschii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9743, 0.9797, 0.9796
Spacegroup name	P 43 21 2
Unit cell lengths	95.254, 95.254, 297.957
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.220 - 2.290
R-factor	0.193
Rwork	0.191
R-free	0.22100
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.330
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	61981
Completeness [%]	99.1	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	298	sodium malonate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.00