1YRJ
Crystal Structure of Apramycin bound to a Ribosomal RNA A site oligonucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.127 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.250, 30.900, 45.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| Rwork | 0.246 |
| R-free | 0.30700 |
| Structure solution method | MIR |
| Starting model (for MR) | 1lc4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.630 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.790 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.073 | 0.170 |
| Number of reflections | 3802 | |
| <I/σ(I)> | 43 | |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 5.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 310 | MPD, sodium cacodylate, NaCl, KCl, MgSO4, pH 6.5, EVAPORATION, temperature 310K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | KCl | ||
| 5 | 1 | 1 | MgSO4 | ||
| 6 | 1 | 1 | H2O |
