1YQU

Escherichia coli purine nucleoside phosphorylase II, the product of the xapA gene

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Detector technology	CCD
Collection date	2002-03-04
Detector	MARRESEARCH
Wavelength(s)	0.811
Spacegroup name	P 32 2 1
Unit cell lengths	71.345, 71.345, 267.537
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 3.100
R-factor	0.2461
Rwork	0.245
R-free	0.26740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pnp
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.126	0.382
Number of reflections	15055
<I/σ(I)>	11	2.8
Completeness [%]	99.9	100
Redundancy	7	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	294	Tris, PEG4K, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 294K