1YNW
Crystal Structure of Vitamin D Receptor and 9-cis Retinoic Acid Receptor DNA-Binding Domains Bound to a DR3 Response Element
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.1808 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.104, 57.049, 73.438 |
| Unit cell angles | 90.00, 110.31, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| Rwork | 0.232 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1KB4 and 1DSZ |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.045 | 0.340 |
| Number of reflections | 18127 | |
| <I/σ(I)> | 31.2 | 2.6 |
| Completeness [%] | 96.3 | 97.5 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | PEG 8000, ammonium citrate, Tris, glycerol, DTT, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 8000 | ||
| 2 | 1 | 1 | ammonium citrate | ||
| 3 | 1 | 1 | Tris | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 1 | DTT | ||
| 6 | 1 | 2 | PEG 8000 | ||
| 7 | 1 | 2 | ammonium citrate | ||
| 8 | 1 | 2 | glycerol |
