1YMM
TCR/HLA-DR2b/MBP-peptide complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-08 |
| Detector | ADSC |
| Wavelength(s) | 1.1 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 137.340, 212.620, 278.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.720 - 3.500 |
| R-factor | 0.276 |
| Rwork | 0.274 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 6.610 | 3.100 |
| Rmerge | 0.103 | 0.041 | |
| Number of reflections | 35129 | ||
| Completeness [%] | 94.5 | 92.8 | 82.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7 | 293 | Na-tartrate, ammonium formate, HEPES, pH 7.0, hanging drop, temperature 293K |
