1YM7
G Protein-Coupled Receptor Kinase 2 (GRK2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 115.162, 82.156, 218.795 |
| Unit cell angles | 90.00, 95.57, 90.00 |
Refinement procedure
| Resolution | 14.980 - 4.500 |
| R-factor | 0.22638 |
| Rwork | 0.224 |
| R-free | 0.27868 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omw |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.881 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.660 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Number of reflections | 24246 | |
| <I/σ(I)> | 21.4 | 2.46 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 3.42 | 3.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 278 | HEPES, phosphoserine, sodium Chloride, Glycerol, PEG 8000, Urea, Dithiothreitol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
