1YFR
crystal structure of alanyl-tRNA synthetase in complex with ATP and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-07-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 173.602, 73.926, 73.976 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.2 |
| Rwork | 0.194 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1riq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.056 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.035 | 0.250 |
| Number of reflections | 50235 | |
| <I/σ(I)> | 20.4 | |
| Completeness [%] | 94.8 | 96.9 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.2 | 298 | PEG 5000 monomethyl ether, NaCl, Tris-Cl, Tris-base, ATP, MgCl2, pH 6.2, VAPOR DIFFUSION, temperature 298K |
