1YFL
T4Dam in Complex with Sinefungin and 16-mer Oligonucleotide Showing Semi-specific and Specific Contact and Flipped Base
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 173 |
| Detector technology | AREA DETECTOR |
| Collection date | 2004-06-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.871, 117.648, 87.865 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 29.400 - 3.090 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q0s |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.200 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Number of reflections | 33149 | |
| <I/σ(I)> | 18.7 | 5.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289 | PEG 6000, MES, ammonium acetate, CaCl2, glycerol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 10 | 1 | 2 | CaCl2 | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | ammonium acetate | ||
| 4 | 1 | 1 | CaCl2 | ||
| 5 | 1 | 1 | glycerol | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | PEG 6000 | ||
| 8 | 1 | 2 | MES | ||
| 9 | 1 | 2 | ammonium acetate |
