1YF8
Crystal structure of Himalayan mistletoe RIP reveals the presence of a natural inhibitor and a new functionally active sugar-binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 273 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8453 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 109.422, 109.422, 309.798 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.23715 |
| Rwork | 0.234 |
| R-free | 0.28712 |
| Structure solution method | MIR |
| Starting model (for MR) | 1pc8 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.227 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.020 | |
| Number of reflections | 26570 | |
| <I/σ(I)> | 11 | 33.6 |
| Completeness [%] | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 298 | NaCl, Ammonium Sulphate, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
