1YF3
T4Dam in Complex with AdoHcy and 13-mer Oligonucleotide Making Non- and Semi-specific (~1/4) Contact
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2003-03-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2826 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.900, 125.800, 73.200 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 28.000 - 2.290 |
| R-factor | 0.221 |
| Rwork | 0.214 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q0s |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | GLRF |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Number of reflections | 29474 | |
| <I/σ(I)> | 22.5 | 9.6 |
| Completeness [%] | 95.7 | 97.3 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 289 | PEG MME 5000, citrate-phosphate, ammonium sulphate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG MME 5000 | ||
| 2 | 1 | 1 | citrate-phosphate | ||
| 3 | 1 | 1 | ammonium sulphate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG MME 5000 | ||
| 6 | 1 | 2 | H2O |
