1YDN
Crystal Structure of the HMG-CoA Lyase from Brucella melitensis, Northeast Structural Genomics Target LR35.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9878 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.272, 86.399, 87.683 |
| Unit cell angles | 90.00, 118.70, 90.00 |
Refinement procedure
| Resolution | 28.940 - 2.300 |
| R-factor | 0.271 |
| Rwork | 0.271 |
| R-free | 0.30400 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.940 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.243 |
| Number of reflections | 97811 | |
| <I/σ(I)> | 13.91 | 0.219 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 3.97 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20% PEG3350, 200 mM CaCl2, and 10 mM DTT., pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
