1YBD
Crystal structure analysis of uridylate kinase from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 158.292, 158.292, 58.741 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.170 - 2.600 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25900 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.200 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 27369 | |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 98.4 | 87.1 |
| Redundancy | 13.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 283 | PEG 3350, Sodium Formate, Glycerol, KCl, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
