1YA7
Implications for interactions of proteasome with PAN and PA700 from the 1.9 A structure of a proteasome-11S activator complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.283 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 255.714, 126.961, 181.494 |
| Unit cell angles | 90.00, 92.63, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.17979 |
| Rwork | 0.180 |
| R-free | 0.22403 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pma |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.464 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 257676 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 294 | PEG 1000, sodium phosphate citrate, LiSO4, imidazole, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
