1Y9G
Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU ULTRAX 18 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-01-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.904, 94.371, 67.617 |
Unit cell angles | 90.00, 107.10, 90.00 |
Refinement procedure
Resolution | 24.050 - 1.870 |
R-factor | 0.151 |
Rwork | 0.149 |
R-free | 0.19473 |
Structure solution method | SIRAS |
RMSD bond length | 0.021 |
RMSD bond angle | 1.865 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.050 | 1.920 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.072 | 0.369 |
Number of reflections | 41994 | |
<I/σ(I)> | 10.1 | 2.2 |
Completeness [%] | 84.9 | 75.58 |
Redundancy | 5.4 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 15% PEG3350, 1 mM sodium cacodylate, 20-100 mM sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |