1Y93
Crystal structure of the catalytic domain of human MMP12 complexed with acetohydroxamic acid at atomic resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99993 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 50.913, 59.552, 53.497 |
| Unit cell angles | 90.00, 115.14, 90.00 |
Refinement procedure
| Resolution | 13.990 - 1.030 |
| R-factor | 0.1566 |
| Rwork | 0.156 |
| R-free | 0.16787 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rmz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.379 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 1.080 |
| High resolution limit [Å] | 1.020 | 1.020 |
| Rmerge | 0.057 | 0.251 |
| Number of reflections | 70833 | |
| <I/σ(I)> | 5.5 | 2.7 |
| Completeness [%] | 97.6 | 84.9 |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Tris, PEG 6000, ACETOHYDROXAMIC ACID, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
