1Y8N

Crystal structure of the PDK3-L2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-08-03
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 65 2 2
Unit cell lengths	120.806, 120.806, 238.587
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.690 - 2.600
R-factor	0.21147
Rwork	0.210
R-free	0.24795
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDK2 PDB entry 1JM6
RMSD bond length	0.016
RMSD bond angle	1.700
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.690	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.053	0.545
Number of reflections	32450
<I/σ(I)>	28.7	3.9
Completeness [%]	100.0	100
Redundancy	9.7	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	soudium citrate, sodium potassium phosphate, sodium chrolide, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K