1Y6R
Crystal structure of MTA/AdoHcy nucleosidase complexed with MT-ImmA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.51418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.570, 69.600, 127.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.800 - 2.200 |
| Rwork | 0.193 |
| R-free | 0.24400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | : 1NC1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.810 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 27476 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | PEG 200, sodium acetate, sodium chloride, cobaltous chloride hexahydrate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
