1Y6K

Crystal structure of human IL-10 complexed with the soluble IL-10R1 chain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9184
Spacegroup name	P 32 1 2
Unit cell lengths	46.440, 46.440, 309.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.520
R-factor	0.233
Rwork	0.229
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j7v
RMSD bond length	0.007
RMSD bond angle	1.360
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.610
High resolution limit [Å]	2.520	2.520
Rmerge	0.037	0.308
Number of reflections	11441
Completeness [%]	84.4	68.7
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	PEG 6000, magnesium chloride, ADA, cymal-6, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K