1Y54

Crystal structure of the native class C beta-lactamase from Enterobacter cloacae 908R complexed with BRL42715

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE
Synchrotron site	LURE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-09-01
Detector	MARRESEARCH
Wavelength(s)	0.966
Spacegroup name	P 21 21 2
Unit cell lengths	44.365, 82.221, 96.156
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.227
Rwork	0.227
R-free	0.29190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.021
Data scaling software	CCP4 ((SCALA))
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.070	2.070
Number of reflections	20669
Completeness [%]		90.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	298	PEG 6000 30%, bicine 100mM, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K