1Y0V
Crystal structure of anthrax edema factor (EF) in complex with calmodulin and pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 317.509, 183.348, 141.812 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 29.870 - 3.600 |
| R-factor | 0.286 |
| Rwork | 0.286 |
| R-free | 0.30700 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.730 |
| High resolution limit [Å] | 3.100 | 3.600 |
| Rmerge | 0.047 | 0.555 |
| Number of reflections | 375293 | |
| <I/σ(I)> | 20 | 2.1 |
| Completeness [%] | 98.0 | 100 |
| Redundancy | 3.8 | 3.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 277 | PEG400, MgCl2, Tris-HCl, pH 8.5, VAPOR DIFFUSION, temperature 277K |
