1XZQ
Structure of the GTP-binding protein TrmE from Thermotoga maritima complexed with 5-formyl-THF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 62 |
| Unit cell lengths | 130.030, 130.030, 113.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.930 - 2.900 |
| R-factor | 0.256 |
| Rwork | 0.250 |
| R-free | 0.30700 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.930 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.047 | 0.280 |
| Number of reflections | 24138 | |
| <I/σ(I)> | 25.08 | 7.3 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | ammonium sulphate, mes, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
