1XZ1
Complex of halothane with apoferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9197 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.610, 181.610, 181.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.21157 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1gwg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.187 |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.820 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.081 | 0.514 |
| Number of reflections | 28090 | |
| <I/σ(I)> | 15.9 | 3.7 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 18.2 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | cadmium sulfate, HEPES, sodium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
