1XXH
ATPgS Bound E. Coli Clamp Loader Complex
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9641, 0.9797, 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 98.450, 106.460, 535.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 3.450 |
Rwork | 0.315 |
R-free | 0.35000 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.530 |
High resolution limit [Å] | 3.450 | 3.450 |
Number of reflections | 73704 | |
<I/σ(I)> | 15.1 | 2.1 |
Completeness [%] | 97.8 | 86.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.95 | 293 | PEG 3350, Sodium Phosphate, Anapoe X-405, pH 7.95, VAPOR DIFFUSION, HANGING DROP, temperature 293K |