1XW9
Drosophila thioredoxin, oxidized, P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.741, 65.507, 62.075 |
| Unit cell angles | 90.00, 113.09, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| Rwork | 0.219 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1auc |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 20371 | |
| <I/σ(I)> | 21.4 | |
| Completeness [%] | 98.4 | 95.6 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 298 | ammonium sulfate, PEG4000, pH 6.0, VAPOR DIFFUSION, temperature 298K |
