1XW7
Diabetes-Associated Mutations in Human Insulin: Crystal Structure and Photo-Cross-Linking Studies of A-Chain Variant Insulin Wakayama
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-23 |
| Detector | BRUKER |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 70.852, 70.852, 70.852 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.100 - 2.300 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zeg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.100 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.065 | 0.066 |
| Number of reflections | 5346 | |
| <I/σ(I)> | 9.7 | 9.6 |
| Completeness [%] | 97.7 | 95.2 |
| Redundancy | 12.9 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 290 | Tris, sodium citrate, acetone, phenol, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 6.20 |
