1XU1
The crystal structure of APRIL bound to TACI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-14 |
Detector | SBC-3 |
Wavelength(s) | 0.98040 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.339, 91.839, 102.268 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.17087 |
Rwork | 0.167 |
R-free | 0.20317 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | APRIL alone pdbcode 1U5Z |
RMSD bond length | 0.009 |
RMSD bond angle | 1.216 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.347 | |
Number of reflections | 44579 | |
<I/σ(I)> | 7.4 | 3.1 |
Completeness [%] | 99.6 | 100 |
Redundancy | 6.4 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | well solution: 70% MPD, 0.1 M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |