1XRK
Crystal structure of a mutant bleomycin binding protein from Streptoalloteichus hindustanus displaying increased thermostability
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9083 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.983, 66.617, 47.179 |
Unit cell angles | 90.00, 117.43, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.500 |
R-factor | 0.17508 |
Rwork | 0.174 |
R-free | 0.19425 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jie |
RMSD bond length | 0.011 |
RMSD bond angle | 1.664 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 36444 | |
<I/σ(I)> | 20.3 | 3.9 |
Completeness [%] | 94.4 | 88.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |