1XQP
Crystal structure of 8-oxoguanosine complexed Pa-AGOG, 8-oxoguanine DNA glycosylase from Pyrobaculum aerophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 72.220, 98.110, 36.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.690 |
| R-factor | 0.18467 |
| Rwork | 0.183 |
| R-free | 0.22479 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.609 |
| Data scaling software | XDS |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.740 | 1.800 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.062 | |
| Number of reflections | 28623 | |
| <I/σ(I)> | 17 | 3.47 |
| Completeness [%] | 96.7 | 90.5 |
| Redundancy | 6.8 | 6.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | PEG 4000, HEPES-NaOH, NaCl, Dithio threitol, Dimethyl sulfoxide, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
