1XO1
T5 5'-EXONUCLEASE MUTANT K83A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Detector | XENTRONICS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.890, 76.810, 120.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.226 * |
| Rwork | 0.226 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1exn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.082 * |
| Total number of observations | 158564 * |
| Number of reflections | 21915 |
| Completeness [%] | 99.6 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | Ceska, T.A., (1993) J.Mol.Biol., 233, 179. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 3 | 1 | reservoir | ammonium sulfate | 35 (%(w/v)) | |
| 4 | 1 | drop | potassium phosphate | 25 (mM) | pH7.5 |
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | dithiothreitol | 1 (mM) | |
| 7 | 1 | drop | 100 (mM) | ||
| 8 | 1 | drop | glycerol | 5 (%(v/v)) |
