1XKT
Human fatty acid synthase: Structure and substrate selectivity of the thioesterase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 150 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-08 |
| Detector | RIGAKU |
| Wavelength(s) | 0.96865, 0.97930, 0.97910, 0.96390 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 104.783, 104.783, 126.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 11.820 - 2.600 |
| R-factor | 0.258 |
| Rwork | 0.258 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 21444 | |
| Completeness [%] | 97.4 | 92.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | Ammonium sulfate, MES, glycerol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
