1XJW
The Structure of E. coli Aspartate Transcarbamoylase Q137A Mutant in The R-State
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-12-30 |
| Detector | UCSD MARK III |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 122.116, 122.116, 155.928 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.710 |
| R-factor | 0.178 |
| Rwork | 0.170 |
| R-free | 0.22300 |
| Structure solution method | Refinement from pre-existing model |
| Starting model (for MR) | 1d09 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 7.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.087 | 0.329 |
| Number of reflections | 35809 | |
| <I/σ(I)> | 10.5 | 2.59 |
| Completeness [%] | 89.0 | 85 |
| Redundancy | 2.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 5.9 | 295 | 12 mg/ml Buffer 50 mM Maleic Acid, 3 mM Sodium Azide 1 mM N-Phosphonacetyl-L-Aspartate, pH 5.9, MICRODIALYSIS, temperature 295K |
