1XFP
Crystal structure of the CDR2 germline reversion mutant of cAb-Lys3 in complex with hen egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8453 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.294, 72.334, 38.766 |
| Unit cell angles | 90.00, 106.74, 90.00 |
Refinement procedure
| Resolution | 20.780 - 1.500 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.017 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 53193 | |
| Completeness [%] | 98.0 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Sodium Formate, sodium-HEPES, sodium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
