1XET
Crystal structure of stilbene synthase from Pinus sylvestris, complexed with methylmalonyl CoA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.465, 111.190, 132.015 |
Unit cell angles | 90.00, 93.13, 90.00 |
Refinement procedure
Resolution | 22.580 - 2.000 |
R-factor | 0.195 |
Rwork | 0.195 |
R-free | 0.22300 |
Structure solution method | isomorphous replacement |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 22.580 |
High resolution limit [Å] | 2.000 |
Number of reflections | 96601 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.8 | 292 | PEG 4000, citric acid, pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |