1XB4

Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Wavelength(s)	.97914
Spacegroup name	P 21 21 21
Unit cell lengths	53.360, 123.660, 140.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 3.100
R-factor	0.273
Rwork	0.327
R-free	0.32700
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.400
High resolution limit [Å]	3.050
Number of reflections	18350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	PEG 8000, Magnesium acetate, glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	PEG 8000, Magnesium acetate, glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K