1X6M
Crystal structure of the glutathione-dependent formaldehyde-activating enzyme (Gfa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.360, 118.860, 95.990 |
| Unit cell angles | 90.00, 97.48, 90.00 |
Refinement procedure
| Resolution | 74.290 - 2.350 |
| R-factor | 0.19348 |
| Rwork | 0.189 |
| R-free | 0.25022 |
| Structure solution method | Se-SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.987 |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.330 | 2.450 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.077 | 0.383 |
| Number of reflections | 49354 | |
| <I/σ(I)> | 8.66 | 2.17 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 1.95 | 1.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | (NH4)2SO4, PEG 400, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
