1WZU

Crystal structure of quinolinate synthase (nadA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-23
Detector	ADSC
Wavelength(s)	1
Spacegroup name	P 63
Unit cell lengths	94.610, 94.610, 62.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.500 - 2.000
R-factor	0.222
Rwork	0.217
R-free	0.26800
Structure solution method	MIRAS
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	81.650
High resolution limit [Å]	1.900
Number of reflections	25173

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	293	18% PEG 600. 0.2M imidazole, 0.1M malate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K