1WY9

Crystal structure of microglia-specific protein, Iba1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-28
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	44.056, 44.056, 99.144
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.610 - 2.100
R-factor	0.217
Rwork	0.217
R-free	0.25100
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.610	2.230
High resolution limit [Å]	2.100	2.100
Number of reflections	6506
Completeness [%]	93.7	75.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1.4M sodium citrate in 100mM HEPES-Na (pH 7.5), VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1.4M sodium citrate in 100mM HEPES-Na (pH 7.5), VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1.4M sodium citrate in 100mM HEPES-Na (pH 7.5), VAPOR DIFFUSION, HANGING DROP, temperature 298K