1WVJ
Exploring the GluR2 ligand-binding core in complex with the bicyclic AMPA analogue (S)-4-AHCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-11-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.811 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 94.412, 59.467, 47.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.080 - 1.750 |
| R-factor | 0.17111 |
| Rwork | 0.169 |
| R-free | 0.21418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m5c |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.599 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.080 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 27472 | |
| <I/σ(I)> | 26.7 | 4.1 |
| Completeness [%] | 99.1 | 96.3 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 279 | PEG 3350, phosphate-citrate buffer, lithium sulfate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
