1WUR
Structure of GTP cyclohydrolase I Complexed with 8-oxo-dGTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-28 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.570, 110.560, 70.530 |
Unit cell angles | 90.00, 105.78, 90.00 |
Refinement procedure
Resolution | 46.690 - 1.820 |
Rwork | 0.206 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.890 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.050 | |
Number of reflections | 103478 | |
<I/σ(I)> | 25 | |
Completeness [%] | 98.5 | 98.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 3.4% PEG400, 0.1M HEPES, 2.0M Ammonium sulfate, 15% glycerol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |