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1WSI

Crystal structure of E.coli RNase HI active site mutant (E48A/K87A/D134N)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL44XU
Synchrotron siteSPring-8
BeamlineBL44XU
Detector technologyIMAGE PLATE
Collection date2003-06-21
DetectorMACSCIENCE
Wavelength(s)0.9
Spacegroup nameP 21 21 2
Unit cell lengths121.120, 80.120, 64.458
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution50.000 - 2.000
Rwork0.217
R-free0.25800
Structure solution methodMOLECULAR REPLACEMENT
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.000
High resolution limit [Å]2.0002.000
Number of reflections42895
Completeness [%]99.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1

219869

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